摘要
目的探讨窄谱中波紫外线(NB-UVB)对寻常性银屑病患者血清和皮损中干扰素-γ(IFN-γ)水平的影响。方法20例寻常性银屑病患者随机分为NB-UVB组和对照组,每组10例。NB-UVB组每周光疗3次,共4周;对照组仅采用常规治疗,不予光疗。治疗前后采用银屑病皮损程度评分(PASI评分)标准评分,以酶联免疫吸附双抗体夹心法(ELISA法)测定血清中IFN-γ水平,以荧光定量聚合酶链反应(FQ-PCR)法分析IFN-γ在银屑病皮损中的表达。选择与银屑病患者的年龄、性别匹配的同期在我院行健康体检的10例健康人为正常对照组。结果NB-UVB组和对照组患者治疗后PASI评分分别为(2.6±2.5)和(7.4±1.3)分与治疗前各自PASI评分分别为(12.4±4.2)和(11.6±4.3)分比较差异均有统计学意义(P<0.05),且治疗后NB-UVB组低于对照组,差异有统计学意义(P<0.05)。NB-UVB组、对照组治疗前血清IFN-γ水平与正常对照组比较,差异均有统计学意义(P<0.05);治疗后NB-UVB组、对照组血清IFN-γ水平与治疗前比较,差异有统计学意义,且NB-UVB组低于对照组,差异有统计学意义(P<0.05)。NB-UVB组、对照组皮损细胞内IFN-γmRNA表达量与正常对照组比较差异均有统计学意义(P<0.05);治疗后两组皮损细胞内IFN-γmRNA表达量较治疗前差异均有统计学意义(P<0.05),且NB-UVB组与对照组比较差异亦有统计学意义(P<0.05)。结论NB-UVB可以调节寻常性银屑病患者血清IFN-γ水平和皮损中IFN-γmRNA的表达,改善患者体内细胞因子的失衡状态,从而达到治疗效果。
Objective To determine the effect of narrow-band ultraviolet B(NB-UVB) on level of IFN-γ in serum and skin lesions in psoriasis vulgaris(PV) patients.Methods Twenty PV patients were randomized into NB-UVB group(receiving phototherapy,3 times/week,4 weeks) and control(receiving conventional therapy without phototherapy),10 in each.Serum IFN-γlevel was determined by the standard scores of PASI and ELISA,the expression of IFN-γ in psoriasis skin lesions by FQ-PCR.Another 10 healthy subjects receiving medical examination in our hospital in the corresponding time period were enrolled as normal control group.Results PASI score decreased significantly in 2 groups after treatment(P<0.05),lower in NB-UVB group(2.6±2.5) than in control group(7.4±1.3).The level of serum IFN-γ decreased significantly in 2 groups after treatment(P<0.05),lower in NB-UVB group than in control group(P<0.05).Conclusion NB-UVB can regulate serum IFN-γ level and IFN-γmRNA expression of PV and improve imbalance of cytokines,to achieve therapeutic effect.
出处
《中国全科医学》
CAS
CSCD
北大核心
2009年第14期1334-1336,共3页
Chinese General Practice
