摘要
以赤潮硅藻小新月菱形藻(Nitzschia closterium var. minutissima)为实验材料,研究了短期内(12h)高浓度CO2(5%CO2)对其胞外碳酸酐酶活性和光合作用的影响,结果显示,高浓度CO2培养导致小新月菱形藻胞外碳酸酐酶活性、叶绿素a和叶绿素c含量明显下降.与通空气培养(0.035%CO2)相比,在短期内(12h)胞外碳酸酐酶活性下降了75.4%,叶绿素a、c含量分别降低了5.6%和7.3%;高浓度CO2培养下最大光化学效率(Fv/Fm)、实际光化学效率(Yield)和光化学淬灭系数(qP)下降,但非光化学淬灭系数(qN)升高.研究结果表明,高浓度CO2对胞外碳酸酐酶活性具有明显的抑制作用,小新月菱形藻通过调整光系统II的能量流动和能量利用效率以适应高浓度CO2的环境.
Carbonic anhydrase activity and chlorophyll a fluorescence parameters were investigated to study the impacts of high CO2 concentration on photosynthesis in Nitzschia closterium var.minutissima in short time (12 h). The results showed that high CO2 concentration led to the evident decline of extracellular carbonic anhydrase activity,Chla and Chlc content. Compared with air-grown cell (0.035%CO2),extracellular carbonic anhydrase activity,Chla and Chlc in high CO2-grown cell were decreased by 75.4%,5.6% and 7....
出处
《广州大学学报（自然科学版）》
CAS
2009年第3期49-53,共5页
Journal of Guangzhou University:Natural Science Edition
基金
国家自然科学基金资助项目(40676079)
关键词
高浓度CO2
碳酸酐酶活性
叶绿素A荧光参数
high CO2 concentration
carbonic anhydrase activity
Chla fluorescence parameters
