纤维连接蛋白诱导人胚胎肺纤维细胞增殖的MAPK信号转导通路研究

Studies on MAPK Signal Transduction Pathway Induced by Fibronectin in Human Fetal Lung Fibroblast Cells

目的探讨纤维连接蛋白(FN)诱导人胚胎肺纤维细胞(HFL1)增殖的丝裂原活化蛋白激酶(MAPK)信号转导通路。方法采用不同浓度FN刺激HFL1,观察细胞外信号调节激酶(ERK)抑制剂PD98059、p38蛋白激酶抑制剂SB203580对FN诱导HFL1增殖作用的影响。用四甲基偶氮唑蓝(MTT)法检测各组细胞增殖情况。结果FN对HFL1诱导增殖作用呈剂量依赖性升高趋势,在50ng/ml时作用显著;PD98059和SB203580可抑制FN诱导的HFL1细胞增殖。结论FN诱导HFL1的细胞增殖可以通过MAPK信号转导途径实现。

Objective To investigate the mitogen-activated protein kinase(MAPK)signal transduction pathway induced by fibronectin(FN) in human fetal lung fibroblast cells (HFL1). Methods The HFL1 was stimulated with different concentrations of FN. The effects of extracellular-signal regulated protein kinase(ERK)inhibitor PD98059 and p38MAPK inhibitor SB203580 on cell growth induced by FN were observed. The growth rate of cells was detected by MTT assay. Results FN enhanced the HFL1 proliferation in a dose-dependent man...