黄鳝Ⅰ型hepcidin抗菌肽基因的诱导表达分析

Expression Analysis of Type Ⅰ hepcidin Gene from Rice Field Eel(Monopterus albus)

采用RT-PCR的方法对健康黄鳝(Monopterus albus)不同组织Ⅰ型抗菌肽hepcidin基因的表达情况及受脂多糖(LPS)注射、嗜水气单胞菌感染后该基因的表达情况进行了分析。结果表明:在正常黄鳝各组织中,该基因在肝脏中表达量最高,其次是肾脏,在心脏、皮肤、脑、血液、小肠、脾脏和胃中的表达量较低,而在肌肉中没有检测到该基因的转录本;LPS注射24h后,各组织里的hepcidin基因表达量都有明显升高;当受到病原菌感染后,在所检测的肝脏、肾脏、皮肤、脑、心脏和脾脏等6个组织中该基因的转录本都在感染后24h后急剧上升。这些结果表明黄鳝的Ⅰ型抗菌肽hepcidin基因可能在黄鳝抵抗外界病原物侵染过程中起着重要作用。

RT-PCR was performed to detect the expression profile of type Ⅰ hepcidin gene of rice field eel(Monopterus albus) in healthy organisms and infected organisms with LPS or pathogenic bacteria.The results demonstrated that hepcidin transcripts are highly abundant in liver,abundant in kidney,less abundant in heart,skin,brain,blood cells,spleen,intestine and stomach,and undetectable in muscle.When challenged with LPS and pathogenic bacteria,Aeromonas hydrophila,up-inducible hepcidin mRNA levels were detected in ...