摘要
Objective To explore expression of endogenous ouabain(EO) in multiple adrenal tumors.Methods Thirty one cases of adrenal tumors and 6 cases of healthy adrenal tissues were selected. The expression of EO in the adrenal tissue was detected with immunohistochemical streptavidin peroxadase conjugated(SP) method.Results Most of EO positive products were localized in cytoplasm of the zona reticularis of human adrenal cortex, and positive products showed to be fine granular. There was no positive signal in the medulla. EO showed on diffused positive in patients with pheochromocytoma accompanied high blood pressure[SBP:(165.22±7.61) mmHg, DBP:(105.52±4.26) mmHg], but there were negative in ones with normative blood pressure[SBP:(118.52±4.58) mmHg, DBP:(83±3.60) mmHg]. The expression of EO was positive in all adrenocortical hyperplasic, adenoma and carcinoma, no matter its high or normative blood pressure. The degree of expression of EO in adrenal tissues was related to the level of BP.Conclusion Expression of endogenous ouabain(EO) in healthy adrenal tissue and adrenal tumors was a valuable morphological and pathophysiological clue for the research on ouabain.
Objective To explore expression of endogenous ouabain(EO) in multiple adrenal tumors.Methods Thirty one cases of adrenal tumors and 6 cases of healthy adrenal tissues were selected. The expression of EO in the adrenal tissue was detected with immunohistochemical streptavidin peroxadase conjugated(SP) method.Results Most of EO positive products were localized in cytoplasm of the zona reticularis of human adrenal cortex, and positive products showed to be fine granular. There was no positive signal in the medulla. EO showed on diffused positive in patients with pheochromocytoma accompanied high blood pressure[SBP:(165.22±7.61) mmHg, DBP:(105.52±4.26) mmHg], but there were negative in ones with normative blood pressure[SBP:(118.52±4.58) mmHg, DBP:(83±3.60) mmHg]. The expression of EO was positive in all adrenocortical hyperplasic, adenoma and carcinoma, no matter its high or normative blood pressure. The degree of expression of EO in adrenal tissues was related to the level of BP.Conclusion Expression of endogenous ouabain(EO) in healthy adrenal tissue and adrenal tumors was a valuable morphological and pathophysiological clue for the research on ouabain.
