摘要
AFLP(amplified fragment length polymorphism) is a very powerful fingerprinting technology. The key of making variety fingerprints is to select specific powerful primers for each crop. A quick and effective procedure for selecting AFLP primers for rice variety fingerprinting was established as the following: (1) Choose3 or more group materials that have close genetic relations. (2) Select potential polymorphic primers from primer pairs that are 2 + 2 primer crosses and same at two ends. (3) Recombine the selected potential polymorphic primers and choosing more polymorphic primers. (4) Add one selecting base at one end to become 2 + 3 or 3 + 2 primers and further selecting more polymorphic primers. Some primers were selected with this procedure, such as M21 P87 and M73P17, with which the fingerprints had more polymorphism and high quality.
AFLP(amplified fragment length polymorphism) is a very powerful fingerprinting technology. The key of making variety fingerprints is to select specific powerful primers for each crop. A quick and effective procedure for selecting AFLP primers for rice variety fingerprinting was established as the following: (1) Choose3 or more group materials that have close genetic relations. (2) Select potential polymorphic primers from primer pairs that are 2 + 2 primer crosses and same at two ends. (3) Recombine the selected potential polymorphic primers and choosing more polymorphic primers. (4) Add one selecting base at one end to become 2 + 3 or 3 + 2 primers and further selecting more polymorphic primers. Some primers were selected with this procedure, such as M21 P87 and M73P17, with which the fingerprints had more polymorphism and high quality.
