番红花红T紫外光度法测定微量NO_2-N的研究

A Study on the Determination of Trace Nitrite-N by Spectrophotometric with Safranin-T

研究了在酸性条件下，亚硝酸盐氮与番红花红Ｔ体系的分光光度测定，提出了１种测定 硝酸盐氮的新方法．在 ２．０ ｍｏｌ／Ｌ的硫酸介质中，体系的最大吸收波长为 ３６５ ｎｍ，表观摩尔吸光 系数为３．１×１０４Ｌ／（ｍｏｌ．ｃｍ），质量浓度在０～１０．０ｍｇ／２５Ｌ范围内遵守比耳定律．此法用于 环境水样中微量亚硝酸盐氮测定，结果与经典的盐酸茶乙二胺比色法基本一致．

Based on the spectrophotometric of nitrite-N and Safranin T system,This paper puts forward a new method for the determination of nitrite. In the present of H2SO4(2.0 mol/L),the maximum absorption wavelength is 365 nm and the apparent molar absorptivity is 3.1 ×104L(mol·cm).Beer's law is obeyed in the range of 0- 10.0 mg/25 L. This method has been applied to the determination of micro-amount of nitrite in environmental water,its results are in agreement with the results obtained by the N-(1-naphthyl) ethylenediaminedily drochloride photometric method.