载脂蛋白E基因多态性与老年心脑血管疾病的相关性探讨

The polymorphism of lipoprotein carrying E gene and the relevant study about cerebrovascular diseases in old persons

目的研究载脂蛋白(apo)E基因与冠心病、脑血栓疾病间的相关程度。方法检测汉族正常人113例、冠心病患者50例及脑血栓患者42例的apoE基因型及等位基因频率。应用聚合酶链-限制性片段长度多态性(PCR-RFLP)法扩增基因,限制性内切酶酶解消化,8%聚丙烯酰胺凝胶电泳,银染显带判定基因型,并计算等位基因频率。结果脑血栓患者apoE4ε/3基因型和4ε等位基因频率显著高于正常对照(P<0.01和P<0.05)。但apoE等位基因多态与冠心病间未发生关联。结论apoE等位基因多态与脑血栓疾病间存在相关。

ObjectiveTo study the relativity of lipoprotein carrying(apo)E gene and coronary diseases,cerebral thrombus diseases.MethodsApoE genotypes and the allele frequency of 113 normal persons with the Han Nationality,50 patients with coronary diseases and 42 patients with cerebral thrombus diseases were tested.Genes were increased using the PCR-RFLP method,the restrictive interior contact enzyme enzymolysis digestion,8% polyacrylamide gel electrophoresis,silver dyes to determine the genotypes,and calculate the allele frequencies.ResultsApoEε4/3 genotype and ε4 allele frequency in cerebral thrombus patiens were obviously higher than those in 113 normal persons with the Han Nationality(P<0.01 or P<0.05).But the apoE allele polymorphism didn't have any connection to coronary diseases.ConclusionThe apoE allele polymorphism was relevant to cerebral thrombus diseases.