pH梯度法制备重酒石酸长春瑞滨脂质体

Loading Vinorelbine Bitartrate in liposomes by pH gradient method

目的优选pH梯度法制备重酒石酸长春瑞滨脂质体的制备工艺。方法采用pH梯度法制备重酒石酸长春瑞滨脂质体,用Sephadex G-50柱层析法测定包封率,考察影响包封率的因素。结果制备重酒石酸长春瑞滨脂质体的最佳条件是药物与磷脂的质量比为1∶30,胆固醇与磷脂质量比为1∶4,脂质体外水相pH值最终调至7.4,孵育温度为30℃,此时的包封率可以达到85%以上。结论用pH梯度法制备重酒石酸长春瑞滨脂质体的包封率较高。

Objective To study the method of preparing Vinorelbine Bitartrate in liposomes by pH gradient method.Methods pH gradient method was used to prepare Vinorelbine Bitartrate 1iposomes.The entrapment rate was examined by Sephadex G-50 minicolumn centrifugation.Results The optimum preparation formulation:drug/PC(mass ratio):1∶30,liposomes were pH 7.4 outside and incubated at 30℃.The encapsulation rate of Vinorelbine Bitartrate in liposomes was above 85%.Conclusion The liposomes were prepared with good encapsulation efficiency by pH gradient method.