水体沉积物中石油污染物的生物降解试验研究

Experiment of biodegradation in the petroleum polluted sediments

研究了高效石油降解菌(邻单胞菌属)对水体沉积物中石油污染物的处理效果,以及微生物接种量、石油烃初始浓度和上层水体中溶解氧浓度对其降解效果的影响。结果表明,微生物接种量越多,石油烃去除率越高,加入30mL浓度为105CFU/mL的菌悬液时,沉积物中石油烃的去除率为34.94%;沉积物中石油烃初始浓度过高或过低均不利于石油烃降解,当试验土样中石油烃浓度为107.15mg/kg时,石油烃的去除率为77.30%;水中溶解氧浓度的提高可以加速石油烃的降解,密闭曝气状态下水中DO为7.09mg/L时,石油烃的去除率为64.95%。

The highly efficient petroleum-degrading bacteria(Plesionmonas sp.)was applied to investigate the degradation of petroleum from the sediments of a reservoir,with the different bacteria biomass,initial concentration of petroleum and DO.The results indicated that petroleum removal rate was related on the biomass.The removal rate of petroleum was 34.94% when the biomass was 30 mL with concentration of 105 CFU/mL.The petroleum biodegradation was not very satisfied when the initial petroleum concentrations were higher or lower in the sediments.The removal rate of petroleum was 77.30% when the concentration of the petroleum in the sediments was 107.15 mg/kg.Increasing the concentration of DO in the water upon the sediments could improve the petroleum degradation,when it was 7.09 mg/L,the removal rate was 64.95%.And the biomass of microorganism can be increased under a higher DO.