摘要
The CD59-coding sequences were obtained from 5 mammals by PCR and BLAST, and combined with the available sequences in GenBank, the nucleotide substitution rates of mammalian cd59 were calcu- lated. Results of synonymous and nonsynonymous substitution rates revealed that cd59 experienced negative selection in mammals overall. Four sites experiencing positive selection were found by using "site-specific" model in PAML software. These sites were distributed on the molecular surface, of which 2 sites located in the key functional domain. Furthermore, "branch-site-specific" model detected 1 positive site in cd59a and cd59b lineages which underwent accelerated evolution caused by positive selection after gene duplication in mouse.
The CD59-coding sequences were obtained from 5 mammals by PCR and BLAST, and combined with the available sequences in GenBank, the nucleotide substitution rates of mammalian cd59 were calcu- lated. Results of synonymous and nonsynonymous substitution rates revealed that cd59 experienced negative selection in mammals overall. Four sites experiencing positive selection were found by using 'site-specific' model in PAML software. These sites were distributed on the molecular surface, of which 2 sites located in the key functional domain. Furthermore, 'branch-site-specific' model detected 1 positive site in cd59a and cd59b lineages which underwent accelerated evolution caused by positive selection after gene duplication in mouse.
作者
GONG YuanYing1,2, PENG MinSheng1,2, ZHOU WeiPing1,3,4 & ZHANG YaPing1,4 1 State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China
2 Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
3 Molecular and Cell Biology, University of Science and Technology of China, Hefei 230026, China
4 Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, China
基金
Supported by the National Basic Research Program of China (973 Program, Grant No. 2007CB411600)
the National Natural Science Foundation of China (Grant Nos. 30621092 and 30430110)
Bureau of Science and Technology of Yunnan Prov-ince, China
