人核糖体蛋白S15a在大肠癌组织中的表达及其意义

Expression and significance of ribosome protein S15a gene in colorectal carcinoma

目的研究人核糖体蛋白S15a(RPS15a)在大肠癌组织中的表达情况及其与大肠癌发生发展的关系。方法大肠癌及癌旁正常组织取自82例术前未经放疗、化疗患者的手术切除标本。利用RPS15a多克隆抗体进行免疫组织化学SP染色,检测RPS15a在大肠癌及正常组织中的表达。结果RPS15a阳性表达率在大肠癌组织中为72.0%(59/82),在癌旁正常组织中为40.2%(33/82),二者间差异有统计学意义(χ2=17.76,P<0.05);在浆膜浸润大肠癌组织中为80.8%(42/52),在无浆膜浸润大肠癌组织中为56.7%(17/30),二者间差异有统计学意义(χ2=5.47,P<0.05);在无淋巴结转移者为58.3%(21/36),在有淋巴结转移者为82.6%(38/46),二者间差异有统计学意义(χ2=5.89,P<0.05)。结论RPS15a在大肠癌组织中呈高表达状态,RPS15a基因可能在大肠癌的发生、发展中起重要作用。

Objective To study the expression and significance of ribosome protein s15a (RPS15a) gene in colorectal carcinoma tissues. Methods SP immunohistochemistry was used to detect the expression of RPS15a protein in 82 samples of colorectal carcinomaand adjacent nonneoplastic tissues. Results In the 82 samples of colorectal carcinoma and adjacent normal tissues, the positive expression rate of RPS15a protein was 72.0% (59/82) and 40.2% (33/82), respectively. Statistical analysis suggested that the positive expression rate of RPS15a protein in the colorectal carcinoma tissues was significantly higher than that in the normal tissues (χ2=17.76, P <0.05). The expression of RPS15a protein was significantly correlated with the depth of invasion (χ2=5.47, P <0.05) and lymph node metastases (χ2=5.89, P < 0.05). Conclusion High expression of RPS15a protein may play an important role in the development of colorectal carcinoma tissues.