维生素C对大鼠急性肺损伤CRP水平的影响

The influence of Vitamin C on CRP of ALI of big rats

目的了解C-反应蛋白(CRP)在大鼠急性肺损伤(ALI)中的水平及维生素C(VC)对CRP的影响。方法将实验动物随机分为6个VC治疗组,A组:油酸+VC后第6 h组;B组:油酸+VC后第8 h组;C组:油酸+VC后第12 h组;D组:油酸+VC后12 h-再+VC-第16 h组;E组:油酸+VC后12 h-再+VC-第24 h组;F组:油酸+VC后12 h-再+VC-24 h后再+VC-第36 h组。用生理盐水替换VC为6个油酸损伤对照组,分别为A′、B′、C′、D′、E′、F′组。用血清丙二醛(MDA)评价氧自由基水平,用CRP水平评价急性炎症反应程度。结果对照组MDA水平明显高于治疗组,MDA峰值出现在损伤后12 h,每次VC干预后MDA均有所下降;CRP随着炎症的进展呈上升趋势,损伤16 h以后上升明显,VC早期(6 h)使CRP水平上升,此后再予以干预对CRP无明显影响。结论维生素C是一种较强的抗氧化药物,能有效降低MDA,早期升高CRP,对ALI的治疗有一定意义。

Objective To understand the level of CRP in acute lung injury(ALI) of big rats and effect to it by VC.Methods xperimental rats were divided into 12 groups randomly.Half were the curative groups and the others were the injured groups as control.The curative groups included Group A: 6hrs after being injected oleic acid and VC;Group B: 8hrs after being injected oleic acid and VC;Group C: 12hrs after being injected oleic acid and VC;Group D: 16hrs,including the 12hrs after being injected oleic acid and VC,and the 4hrs after being annexed VC again;Group E: 24hrs,including the 12hrs after being injected oleic acid and VC,and the 12hrs after being annexed VC again;Group F: 36hrs,including the 24hrs as Group E and the 12hrs after being annexed VC one more time.The injured groups as control: VC was replaced by NS in each group as described above,and the new groups were marked as A′,B′, C′,D′,E′.the level of oxygen free radicals was assessed by examining malondialdehyde(MDA),degree of ALI was evaluated by level of CRP.Results The level of MDA in injured groups was obvious higher than the curative groups,the peak value appeared in Group C′,decreased after using VC.CRP appeared higher and higher with the increase of inflammation,especially after 16hrs,VC could increase CRP(6 h),but not in any other groups.Conclusion Vitamin C was a kind of fairly strong anti-oxygenic medicine,it could decrease MDA and increase CRP.