通心络胶囊对血管性痴呆小鼠脑血管储备能力的影响

The Effect of Tong-xin-luo Capsule on the Cerebrovascular Reserve Capacity in Vascular Dementia Mice

目的观察通心络胶囊对血管性痴呆(VD)小鼠脑血管储备功能的影响。方法用夹闭双侧颈总动脉,同时腹腔注射硝普钠制做VD小鼠模型。通心络灌胃(0.2g·kg-1·d-1)处理小鼠,生理盐水作为对照。18只小鼠随机分为假手术组(对照组)、模型加生理盐水组(安慰剂组)、模型加通心络组(治疗组)。处理30d后应用RT-PCR和免疫印迹方法测定小鼠海马部位血管内皮细胞生长因子(VEGF)和一氧化氮合酶(eNOS)的表达。结果(1)RT-PCR:对照组与安慰剂组的eNOS基因mRNA表达无明显差异,而治疗组eNOS mRNA表达明显增强,与安慰剂组比较差异有统计学意义(P<0.01)。治疗组VEGF mRNA表达明显高于安慰剂组,差异有统计学意义(P<0.01);(2)Western Blot:安慰剂组的eNOS和VEGF蛋白表达高于对照组(P<0.05),治疗组eNOS和VEGF蛋白表达明显上调,高于安慰剂组,差异有统计学意义(P<0.01)。结论通心络能明显增强VD模型大鼠海马组织VEGF和eNOS表达,提高其脑血管储备能力,从而减轻VD模型小鼠的缺血性脑损伤。

Objective To study the effect of Tong-xin-luo capsule on the cerebrovascular reserve capacity in vascular dementia(VD)mice.Methods VD mode1 was established by occlusion of the bilateral common carotid arteries plus intraperitoneal injection of sodium nitroprusside,intragastric administration of Tong-xin-luo 0.2 g.kg-1.d-1?or physiologic saline after making model.Eighteen mice was randomly divided into sham operated group(control group),model group plus physiologic saline(placebo group)and model group plus Tong-xin-luo(treatment group).VEGF and eNOS expression in hippocampa1 structure of mice brain by using the methods of RT-PCR and Western Blot were measured after making model,behind thirty days to drug.Results(1)RT-PCR:There is no significantly difference of eNOS mRNA expression between control group and placebo group,but in Tong-xin-luo group,eNOS and VEGF mRNA expression are much higher than placebo group(P<0.01);(2)Western Blot:In comparison with control group,eNOS and VEGF protein expression of placebo group much higher(P<0.05),while eNOS and VEGF protein expression of Tong-xin-luo group increased significantly when compared with placebo group(P<0.01).Conclusion The Tong-xin-luo capsule can increase the level of VEGF and eNOS in hippocampal structure of VD model mice brain,thus decrease the vascular injury and increase the cerebrovascular reserve capacity of VD model mice.