癌胚抗原mRNA指标诊断消化系统肿瘤血液转移的研究

A Study on Detection of Carcinoembryonic Antigen MRNA in Blood for Diagnosis of Haemato-translocation of Digestive System Tumor

目的分析检测血液中癌胚抗原信使核糖核酸(CEA mRNA)诊断消化系统肿瘤血行转移的应用价值。方法以41例消化系统肿瘤患者(肿瘤组)和32例非肿瘤性消化道疾病患者(对照组)为对象,应用逆转录-套式-聚合酶链反应技术检测血液标本CEA mRNA。结果肿瘤组的CEA mRNA阳性结果(20例)远高于非肿瘤组(1例)(P<0.001),CEA mRNA指标诊断消化系统肿瘤血行转移的特异性和敏感性分别为96.9%和48.8%。结论CEA mRNA是一个实用价值较高的诊断消化系统肿瘤血行转移指标,检测血液CEA mRNA有助于了解肿瘤血行转移。

Objective The aim was to acquaint the value on detection of carcinoembryonic antigen mRNA(CEA mRNA)in blood for to diagnose haemato-translocation of digestive system tumor.Methods There were 41 patients with digestive system tumor and 32 patients with non-tumor digestive system diseases in the study.CEA mRNA in blood was detected by reverse transcriptase-nested primers-polymerase chain reaction.Results The positive result of CEA mRNA in the tumor group(20/41)was far high than the non-tumor group(1/32)(P<0.001).The specificity and sensitivity of CEA mRNA in diagnosis of haemato-translocation of digestive system tumor was 96.9% and 48.8%.Conclusion CEA mRNA is a useful index for diagnosis of haemato-translocation of digestive system tumor.