甲醛喷雾吸入急性肺水肿大鼠血浆总抗氧化能力的变化

Studies on the changes of total antioxygen capacity in plasma of acute pulmonary edema rats model induced by breathe in pulverized formaldehyde

目的探讨甲醛喷雾吸入诱导大鼠急性肺水肿后血浆总抗氧化能力的变化。方法大鼠随机分为对照组和甲醛组,甲醛组大鼠放入鼠笼并置于正方形染毒箱(0·4m×0·4m×0·4m)中盖好,四角放置200g钠石灰,加氧泵连接导管插入盛有200ml的40%甲醛原液的1000ml烧杯通气,每隔25min向箱内喷入甲醛雾化汽一次,让大鼠持续吸入高浓度甲醛诱发急性肺水肿,2h后取出大鼠置于箱外通风处,5h后眼球取血肝素抗凝测定血浆总抗氧化能力,大鼠处死称体重及心、肺重量,计算心肺系数和体肺系数。生理盐水对照组大鼠处理同上,但甲醛换为生理盐水。结果甲醛组肺肿大,边钝圆,切开可见粉红色液流出;与生理盐水对照组比较,甲醛组心肺系数显著增大(2·55±0·32比1·58±0·10,P<0·01),肺指数也显著增大(9·49±1·19比4·67±0·34,P<0·01),血浆T-AOC明显升高(13·8±4·1比7·2±1·2,P<0·01)。结论甲醛吸入后肺水肿形成,大鼠血浆总抗氧化能力升高并可能与应激有关。

Objective To study the change of acute pulmonary edema rats induced by breath in atomizated formaldehyde. Methods The rats were randomly dived into saline group and formaldehyde group, formaldehyde group rats which inside the mouse cage were put into the cube box(length 0.4rn) and prestore 200 g dry sodalime in the each corner of the cage,one 1 000 ml capacity beakers which fill with 200 ml 40% formaldehyde was put inside the cage and the oxygen to be applied by a plastic tube which connected with an electric pump.Rats breathed in formaldehyde for 2 hours (during the experiment, the atomizated formaldehyde was spouted inside the cage per 25 min) and then were picked out.Rats were killed 5 hours later.The lungs and the hearts to be weighted, the total antioxygen capacity(T-AOC) of plasma to be measured.The saline group rats were treated as above but the formaldehyde to be instead of saline.Results The pathological anatomy showed that pulmonary edema, hemorrhage and fill with some pink fluid in the formaldehyde inhabitation group.Compare with saline group, the index of Lung/Body Weigh(P<0.01) and Lung/Heart Weigh(P<0.01) increased, the T-AOC of plasma (P<0.01) increased significantly too in the formaldehyde group.Conclusion Breathing in atomizated formaldehyde can induced acute pulmonary edema, the total antioxygen capacity of plasma increased and probably relevant to Stress.