HPLC法测定循环康胶囊中人参皂苷Rg_1、Rb_1的含量

Detemination of ginsenoside Rg_1,ginsenoside Rb_1 In xunhuankang Capsules by HPLC

目的 建立HPLC法测定循环康胶囊中人参皂苷Rg1、Rb1含量的方法.方法 采用Shim-pack VP-ODS-C18色谱柱(150 mm×4.6 mm,5 m),以乙腈(A)和水(B)为流动相梯度洗脱,流动相梯度:0 min(20%A)→15 min(40%A)→20 min(20%A)→25 min(20%A).流速:1.0 ml/min,柱温:30℃,检测波长:203 nm.结果 人参皂苷Rg1、Rb1线性范围分别为2.45～12.25 g、2.65～13.25 g.该方法加样回收率:人参皂苷Rg1为96.1%、人参皂苷Rb1为94.9%,RSD分别为2.0%、1.8%.结论 该方法操作简便、准确、可靠,精密度好,可用于循环康胶囊的含量测定.

Objective To establish an HPLC method for the determination of ginsenoside Rg1,ginsenosigeRb1 in xunhuankang Capsulese.Methods An HPLC method was applied with a Shim-pack VP-ODS-C18(150×4.6 mm,5m)column by a gradient elution using (A)acetonitrile-(B)water as the mobile phase 0 min(20%A)→15 min (40%A) →20 min(20%A) →25 min(20%A).The flow rate was 1.0ml·min-1.The column temperature was 30℃ and the UV detection wavelength was203nm.Results The linearity range of ginsenoside Rg1and ginsenosigeRb1 is 2.45～12.25 g、2.65～13.25 g.the recovery of ginsenoside Rg196.1%,ginsenosigeRb194.9%,and RSD is 2.0%,1.8%.Conclusion This method is simple,exact,leliable and precise.It is used to measure /determine the content of xunhuanKang Capsulese Oral Solusion.