壬基酚对小鼠睾丸NOS-1表达的影响

Effect of nolyphenol on the expression of NOS-1 in mice testis

目的研究壬基酚食入对雄性小鼠睾丸组织NOS-1表达的影响,探讨壬基酚对雄性小鼠生殖功能影响机制。方法健康雄性昆明小鼠30只,随机分为A、B、C3组,A组为对照组;B组为1%壬基酚实验组;C组为1‰壬基酚实验组。10d后处死各组小鼠,取出睾丸,Zamboni固定液固定,常规冰冻制片,免疫组织化学染色,光镜观察。结果B组和C组小鼠睾丸组织NOS-1阳性表达同对照组比较明显增强,B组和C组间未见明显差异。结论壬基酚喂食后,对雄性小鼠生殖功能的损伤作用,可能通过NOS-1的变化而造成。

Objective To study the changes of the expression of NOS-1 in testis of male mice which drunk different dose nolyphenol solution,and discuss the effect of nolyphenol on reproductive function of male mice.Methods Take 30 healthy male mice of Kunming Genus,divide them into three groups at random:A group,B group and C group.A group is control group;B group is 1% test group;C group is 1‰ test group.On the 10th day,quickly take out each group' testis;fix in the Zamboni fixation solution,conventionally produce frozen section and stain section by immunohistochemical technology.Results The expression of NOS-1 in test groups increase,there is no obvious change in different dose groups.Conclusion After mice was fed nolyphenol,the expression of NOS-1 visibly increased.So it clue to that harmful effect on the reproductive function of male mice can own to change of NOS-1 after feeding nolyphenol.