膀胱癌患者CYP1A1基因MSPI多态性的相关分析

Analysis of the polymorphism of CYP1A1-MSPI gene in patients with bladder cancer

目的探讨细胞色素CYP1A1基因MSPI多态性与膀胱癌遗传易感性的关系。方法应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)分析技术,检测44例膀胱癌患者(病例组)和85例同期住院非膀胱癌患者(对照组),检测CYP1A1基因MSPI多态性位点的3种基因型及等位基因的分布频率。结果在病例组CYP1A1基因MSPI位点基因型分布频率为:TT(54·5%)、TC(36·4%)、CC(9·1%),等位基因分布频率为T(72·7%)、C(27·3%);在对照组CYP1A1基因MSPI位点基因型的分布频率为TT(61·2%)、TC(31·2%)、CC(7·1%),等位基因分布频率为T(77·1%)、C(22·9%)。各个基因型在两组中所占的比例差异无统计学意义(P>0·05);T、C等位基因频率两组比较差异亦无统计学意义(P>0·05)。结论CYP1A1基因MSPI位点多态性的单独存在可能与本地区膀胱癌易感性无关。

Objective To discuss the relationship between the polymorphism of cytochrome CYP1A1-MSPI gene and genetic predisposition of bladder cancer.Methods The distribution frequence of allelic and three genotype of CYP1A1-MSPI polymorphism site of 44 patients with bladder cancer(case group) and 85 patients without bladder cancer(control group) by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) were detected.Results The distribution frequences of genotype of CYP1A1-MSPI site of the case group were TT(54.5%),TC(36.4%),CC(9.1%),and the frequences of allelic gene were T(72.7%),C(27.3%).While the distribution frequences of genotype of CYP1A1-MSPI site of the control group were TT(61.2%),TC(31.2%),CC(7.1%),and the of allelic gene were T(77.1%),C(22.9%).The ratio of genotype in each group has no significant difference(P>0.05);the frequence of allelic gene of T and C in each group also had no significant difference(P>0.05).Conclusion There may be no relationship between the individualism of CYP1A1-MSPI site polymorphism and susceptibility of bladder cancer in this area.