CoCl_2所致化学缺氧促进脂肪组织缺血缺氧耐受相关基因表达的研究

Study on the express of HIF-1α protein and HIF-1α mRNA afterin jected CoCl_2

目的探讨脂肪组织中是否存在缺氧预适应现象,构建CoCl2致缺氧的缺氧模型。方法不同浓度的CoCl2大鼠腹股沟区皮下层浸润注射,分别用免疫组织化学法和RT-PCR法检测该区脂肪HIF-1α蛋白和HIF-1αmRNA的表达情况。结果用不同浓度的CoCl2行化学缺氧后在不同时间点观察有不同程度的HIF-1α蛋白和HIF-1αmRNA表达,其中,在浓度为20～40mg/kg,时间为3h时脂肪组织中HIF-1α表达达高峰。结论脂肪组织存在缺氧预适应现象;CoCl2的浓度选择20～40mg/kg,时间选择3h时缺氧预适应效果较佳。

Objective To investigate whether hypoxia preconditioning exist in adipose tissue,and construct the hypoxemic model of adipose tissue.Methods After injected different density CoCl2 to rat's subcutaneously of groin,use immunohistochemical method detect HIF-1α protein,and use RT-PCR detect HIF-1α mRNA.Results After injected different density CoCl2,the adipose tissue of rat's groin express different degree of HIF-1αprotein and HIF-1αmRNA.,and reach the peak when the density is 20-40 mg/kg and the time is 3 h.Conclusion Hypoxia preconditioning exist in adipose tissue,and the effect of preconditioning is most evidence when the density is 20-40 mg/kg and the time is 3 h.