毛细管柱气相色谱法测定冠心苏合胶囊中的冰片含量

Capillary column gas chromatographic determination of borneol in guanxinsuhe capsules

目的 建立冠心苏合胶囊中冰片的含量的测定方法 .方法 以水杨酸甲酯为内标物,采用内标法测定;选择FID检测器、SE-54毛细管柱气相色谱法测定冠心苏合胶囊中冰片的含量.结果 在该色谱条件下,样品中冰片与内标物能得到很好的分离,冰片在0.2～2 mg/ml范围内线性关系良好(r=0.999 7,n=6),加样回收率为96.93%(RSD=1.1%,n=5).结论 本方法 灵敏、准确、重现性好,可用于该制剂中的冰片含量测定.

Objective To establish a GC method for determing the contents of borneol in guanxinsuhe capsules.Methods Applying internal standard method with Methyl salicylate as internal substance.Using GC method for quantitative analysis of borneol with SE-54 capillary column,FID detector.Results The components exhibit an excellent separability and the borneol have an good linearity in the rang of 0.2-2.0 mg/ml(r=0.999 7,n=6),with a mean recovery of borneol as high as 96.93%(RSD=1.1%,n=5).Conclusion This method is sensitive,accurate and reproducible,and can be used to determing the borneol in guanxinsuhe capsules.