摘要
目的探讨血小板相关抗体检测及血小板交叉配型对血小板输注效果的影响。方法采用酶联免疫竞争抑制(ELISA)法对临床确认的特发性免疫性血小板减少症(ITP)106例、系统性红斑狼疮(SLE)21例、甲状腺机能亢进症40例、非免疫性血小板减少症(NATP)25例及正常对照组80例进行检测;采用简易致敏红细胞血小板血清学技术(SEPSA)对部分血小板相关抗体阳性病例进行血小板交叉配型。结果各种免疫性血小板减少症患者血小板相关抗体(PAIg)阳性率较高,与正常对照组相比差异有统计学意义(P<0.05),非免疫性血小板减少组与免疫性血小减少组相比前者阳性率增高不明显。对30例血小板相关抗体阳性且输注无效的患者进行血小板配型输注,其中25例相合,有效率为83%。结论血小板相关抗体检测具有重要的临床应用价值,血小板配型可有效提高临床输注效果。
Objective To explore the platelet relative antibody has diagnosis value and effect of platelet crossmatch.Methods 106 ITP,21 SLE,40 hyperethyreosis,25 NATP and 80 mormal contrast were detected by ELISA.Meanwhile RP were detected by flow cytometry in ITP and mormal contrast.Crossmatch approach was used to screen compatible plateler for some pstients wirh platelet relative antibody positive.Results Various kinds of immunity platelet-penia had higher positive rates in PAIg than those in normal controls (P<0.05).The positive ratio and various dinds of indexes were lower in non-immunity platelet-penia growps than those in immunity platelet-penia growps.30 cases were selected to use crossmatch,with platelet relative antebody positive and refractoriness to platet transfusion,and the effective rate was 83%.Conclusion Detection of platelet relative antibody has diagnosis value.Platet crossmatch can raise the effect of clinical transfusion.
出处
《中国实用医药》
2007年第35期10-12,共3页
China Practical Medicine
