复方三叶香茶菜抗大鼠肝纤维化的实验研究

Mechanism of the preventive effect of a compound Chinese drug of SanYeXiangChaCai on carbon tetrachloride induced hepatic fibrosis

目的研究复方三叶香茶菜(三叶香茶菜、黄根、三七)的保肝抗肝纤维化作用及其机制。方法运用腹腔注射四氯化碳致大鼠肝纤维化模型,观察复方三叶香茶菜对大鼠肝纤维化的影响。运用苏木素-伊红染色、Massion染色检测肝纤维化的变化,生化检测肝脏羟脯氨酸、血清丙氨酸氨基转移酶(ALT)及天冬氨酸转移酶(AST),免疫组织化学法观察α-平滑肌肌动蛋白(α-SMA)的表达,分别应用RT-PCR和Western blot法检测转化生长因子β1(TGF-β1)及结缔组织生长因子(CTGF)mRNA和蛋白质表达状况。结果复方三叶香茶菜能显著改善四氯化碳诱导的肝纤维化损伤,肝组织羟脯氨酸含量显著降低,血清ALT和AST含量明显低于肝纤维化模型组,肝组织TGF-β1及CTGF的mRNA和蛋白质表达均明显下降。结论复方三叶香茶菜能有效地抑制四氯化碳诱导的肝纤维化大鼠的肝损伤及纤维化进程,其主要机制可能与复方三叶香茶菜显著抑制TGF-β1和CTGF的表达有关。

Objective To examine the effects of a compound Chinese medicine of SanYeXiangChaCai (Rabdosia ternifold, Prismatomeris tetrandra, Panaxpseudo-ginseng) on the treatment of hepatic fibrosis, and to explore its mechanisms in rat model. Methods The rat model of carbon tetrachloride (CCl4)-induced hepatic fibrosis was used to assess the effect of h SanYeXiangChaCai on the treatment offibrosis. Hepatic fibrosis was evaluated by hematoxylin-eosin staining and Masson's trichrome staining. Hepatic hydroxyproline and serum ALT/AST were examined by biochemistry. The expression of α-SMA in liver tissue was examined by immunohistochemistry. The expression of TGF-β1 and CTGF was assessed by RT-PCR and Western blot analyses. Results Histological and hepatic hydroxyproline examination revealed that SanYeXiangChaCai significantly arrested progression of hepatic fibrosis. SanYeXiangChaCai caused significant amelioration of liver injury (reduced activities of serum ALT and AST) and decreased α-SMA expression. Furthermore, SanYeXiangChaCai markedly reduced both mRNA and protein expression of TGF-β1 as well as CTGF. Conclusions These results suggest that SanYeXiangChaCai ameliorates carbon tetrachloride-induced hepatic fibrogenesis in rats via inhibition of TGF-β1 and CTGF expression.