转化生长因子β1对大鼠肝卵圆细胞增殖和凋亡的影响

Influence of TGF-β1 on rat hepatic oval cell proliferation and apoptosis

目的研究转化生长因子-β1(TGF-β1)在肝卵圆细胞增殖过程中发挥的调控作用。方法60只雄性SD大鼠随机分成实验组和对照组,建立肝卵圆细胞增殖动物模型(2-AAF/PH),采用免疫组织化学、RT-PCR、原位细胞凋亡检测方法,观察上述两组动物模型不同时间点肝脏卵圆细胞增殖情况和TGF-β1及其受体动态表达变化。结果实验组在肝切除术后2 d即出现卵圆细胞,10 d达高峰,16 d卵圆细胞数目明显减少,肝小叶和肝索结构恢复;TGF-β1表达在术后6 d开始增多,16 d达高峰,21 d降至正常水平;TUNEL染色阳性细胞高峰出现在卵圆细胞的增殖高峰后期,和TGF-β1表达高峰出现在同一时期。结论TGF-β1与卵圆细胞增殖和凋亡密切相关,在肝卵圆细胞增殖过程中起负调控作用。

Objective To study the regulation of TGF-β1 on rat hepatic oval cells(HOCs) proliferation.Methods 60 male Sprague-Dawley rats were randomly divided into the control group and the experimental group,and were established animal models of HOCs proliferation(2-AAF/PH).Using immunohistochemistry,RT-PCR and in situ detection of apoptosis,HOCs proliferation and dynamic expression of TGF-β1 of two model sets were observed at different time points.Results HOCs of the experimental group appeared on the second day after hepatectomy,and reached the peak on day 10.On day 16 oval cells significantly reduced and the structures of lobules of liver and hepatic cord recovered.TGF-β1 expression began to increase on day 6 after hepatectomy and reached its peak on day 16,and then it dropped to the normal level on day 21.TUNEL-positive cells in the experimental group reached its peak on day 16,in the late stage of the proliferation of oval cell's peak and in the same period of the expression of TGF-β1's peak.Conclusion TGF-β1 is closely related to HOCs proliferation and apoptosis,and it has negative regulated in HOCs proliferation.