细胞免疫在不同纲(罗非鱼/大鼠)肝细胞移植排斥反应中的作用

The role of cellular immunity in the xenotransplantation of hepatocytes in donor and recipient combination of tilapia to rat

目的探讨罗非鱼到大鼠肝细胞移植的细胞免疫排斥反应机制。方法SD大鼠随机分为移植组和对照组,实验组脾内移植2×107个罗非鱼肝细胞,对照组脾内注射生理盐水。术后以HE染色法观察移植物组织学变化,SABC免疫组化法检测移植物周围大鼠CD4、CD8阳性细胞。结果肝移植术后2 h罗非鱼肝细胞形态完整,4 h出现部分肝细胞边界不清,核固缩、溶解,8 h后很难见到正常肝细胞。移植后4 h移植物周围可见CD4和CD8阳性细胞聚集。结论在罗非鱼肝细胞移植到大鼠脾内诱发的排斥反应中,细胞免疫可能具有重要作用。

Objective To investigate the rejection mechanisms of cellular immune in xenogeneic hepatocytes transplantation between tilapia and rat.Methods The outbred SD rats were divided randomly into two groups,the control group and transplantation group.And the tilapia(donor) hepatocytes(2×107) were transplanted into the spleen of the rat(recipient) in the transplantation group,normal sodium was injected into the spleen of the rat(recipient) in the control group.The histological characteristics of transplanted tilapia hepatocytes were observed by HE staining.The CD4/CD8 lymphocytes around the grafts were detected with immunohistochemistrical method.Results Grafted hepatocytes disappeared quickly after transplantation.Only a few living hepatocytes could be found in spleens after 8 hours.The CD4/CD8 lymphocytes were congregated around the grafts at 4 hours post-graft.Conclusion The cellular immune responses participate in the rejection of xenogeneic hepatocytes transplantation between tilapia and rat.