高效液相色谱法测定栀子金花丸中大黄素和大黄酚的含量

The highly effective liquid phase determination zhizijinghua pill of Emodin and Chrysophanol’s content

目的建立栀子金花丸中大黄素和大黄酚的含量测定方法。方法采用RP-HPLC法进行测定,色谱柱为DikmaC18(5μm,250mm×4.6mm);流动相为甲醇-水-冰醋酸(80∶20∶1);检测波长为254nm:柱温:25℃,流速:1.0ml/min。结果栀子金花丸中大黄素和大黄酚的线性范围为3.15～31.5μg/ml,7.00～70.0μg/ml,r=0.9998,r=0.9998,平均回收率为99.20%,99.30%,RSD值为0.4%,0.2%。结论该方法简便,结果准确,重现性好,可作为栀子金花丸的质量控制方法。

Objective Establishes with the opposition highly effective liquid chromatography (RP-HPLC)determination zhizijinghua pill of Emodin and Chrysophanol’s content method.Methods Uses RP-HPLC to dectect,chromatograph volume was Dikma C18 (5 μm,250 mm×4.6 mm),mobilephase:methanol-water-glacialaceticacid(80∶20∶1); dectectwavelength is 254 nm;the temperature of chromatopraph volume was 25℃,flow rate:1.0 ml/min.Results The emodin linear scope 3.15～31.5 μg/ml (r=0.999 8),the Chrysophanol liner scope 7.00～70.0 μg/ml (r=0.999 8).the average recoveries were 99.20%,99.30% and the RSD were 0.4%,0.2%.Conclusion These methods can be used for quality control of zhizijinghua pill.