摘要
Our previous research results have shown that the Ti-O films with appropriate characteristics possess great potentials for biomaterials application. In this paper, using plasma immersion ion implantation and deposition (PIII-D), titanium oxide thin films are fabricated onto silicon wafer. The antithrombogenesis of films is evaluated in vitro through the platelet adhesion investigation. The biological behavior of human umbilical vein en-dothelial cells (HUVEC) on the film surface is investigated in vitro by endothelial cell (EC) culture. Our results reveal that the crystalline Ti-O films exhibit attractive blood compatibil-ity. The in vitro HUVEC-cultured investigation of Ti-O film surface has justified that the biological behavior of HUVECs on different structure surfaces is significantly different. The adherence, growth and proliferation of HUVECs to the crystalline Ti-O film surface are in order, by forming a perfect single layer, preserving the natural original shape and dis-playing the cobblestone road metal rank, and obviously superior to that on the amorphous Ti-O film surface. According to our study, the crystalline Ti-O film, with proper microstruc-ture, is helpful for seeding ECs and can be used as a functional surface for the adherence and growth of ECs.
Our previous research results have shown that the Ti-O films with appropriate characteristics possess great potentials for biomaterials application. In this paper, using plasma immersion ion implantation and deposition (PIII-D), titanium oxide thin films are fabricated onto silicon wafer. The antithrombogenesis of films is evaluated in vitro through the platelet adhesion investigation. The biological behavior of human umbilical vein endothelial cells (HUVEC) on the film surface is investigated in vitro by endothelial cell (EC) culture. Our results reveal that the crystalline Ti-O films exhibit attractive blood compatibility. The in vitro HUVEC-cultured investigation of Ti-O film surface has justified that the biological behavior of HUVECs on different structure surfaces is significantly different. The adherence, growth and proliferation of HUVECs to the crystalline Ti-O film surface are in order, by forming a perfect single layer, preserving the natural original shape and displaying the cobblestone road metal rank, and obviously superior to that on the amorphous Ti-O film surface. According to our study, the crystalline Ti-O film, with proper microstructure, is helpful for seeding ECs and can be used as a functional surface for the adherence and growth of ECs.
作者
CHEN Junying1, WAN Guojiang1, LENG Yongxiang1, YANG Ping1, SUN Hong1, WANG Jin1, HUANG Nan1, CHEN Huaiqing2 & TANG Rong2 1. College of Materials Science and Engineering, Key Laboratory of Advanced Materials Processing of Chinese Education Ministry, Southwest Jiaotong University, Chengdu 610031, China
2. School of Basic Medical Sciences, West China Center of Medical Sciences, Sichuan University, Chengdu 610064, China
基金
This work was supported by the Key Basic Research Project (Grant Nos. G1999064705 and 2005CB623904)
the National Natural Science Foundation of China (Grant Nos. 30300087 and 30318006).
