摘要
The corpus luteum (CL) is a transient endocrine organ that secretes progesterone to support early pregnancy. If implantation is unsuccessful, luteolysis is initiated. Extensive tissue re-modeling occurs during CL formation and luteolysis. In this study, we have studied the possible in-volvement of MMP-2, -9, -14, and their inhibitors, TIMP-1, -2, -3 in the CL of cycling rhesus monkey at various stages by in situ hybridization, immunohistochemistry and microscopic assessment. The re-sults showed that the MMP-2 mRNA and protein were mainly expressed in the endothelial cells at the early and middle stages of the CL development, while their expressions were observed in the luteal cells at the late stage during luteal regression. MMP-9 protein was detected in the CL at the early and middle stages, and obviously increased at the late stage. The expressions of MMP-14 and TIMP-1 mRNA were high at the early and late stages, and low at the middle stage. TIMP-2 mRNA was high throughout all the stages, the highest level could be observed at the late stage. The TIMP-3 produc-tion was detected throughout all the stages, but obviously declined during CL regression. MMP-9, -14 and TIMP-1, -2, -3 were mainly localized in the cytoplasm of the steroidogenic cells. The results suggest that the MMP/TIMP system is involved in regulation of CL development in the primate, and the coordinated expression of MMP-2, -14 and TIMP-1, -3 may have a potential role in the CL forma-tion and the functional maintaining, while the interaction of MMP-2, -9, -14 and TIMP-1, -2, -3 might also play a role in CL regression at the late stage of CL development in the primate.
The corpus luteum (CL) is a transient endocrine organ that secretesprogesterone to support early pregnancy. If implantation is unsuccessful, luteolysis is initiated.Extensive tissue remodeling occurs during CL formation and luteolysis. In this study, we havestudied the possible involvement of MMP-2, -9, -14, and their inhibitors, TIMP-1, -2, -3 in the CLof cycling rhesus monkey at various stages by in situ hybridization, immunohistochemistry andmicroscopic assessment. The results showed that theMMP-2 mRNA and protein were mainly expressed inthe endothelial cells at the early and middle stages of the CL development, while their expressionswere observed in the luteal cells at the late stage during luteal regression. MMP-9 protein wasdetectedin the CL at the early and middle stages, and obviously increased at the late stage. Theexpressions of MMP-14 and TIMP-1 mRNA were high at the early and late stages, and low at the middlestage. TIMP-2 mRNA was high throughout all the stages, the highest level could be observed at thelate stage. The TIMP-3 production was detected throughout all the stages, but obviously declinedduring CL regression. MMP-9, -14 and TIMP-1, -2, -3 were mainly localized in the cytoplasm of thesteroidogenic cells. The results suggest that the MMP/TIMP system is involved in regulation of CLdevelopment in the primate, and the coordinated expression of MMP-2, -14 and TIMP-1, -3 may have apotential role in the CL formation and the functional maintaining, while the interaction of MMP-2,-9, -14 and TIMP-1, -2, -3 might also play a role in CL regression at the late stage of CLdevelopment in the primate.
基金
supported by WHO/Rockefeller Foundation Project,National Natural Science Foundation of China(Grant Nos.90208025,30370200,30270196&30170452)
Knowledge Innovation Program of CAS(Grant No.KSCX2-SW-201).
