摘要
The mammal's high elevation(hypoxia) adaptation was studied by using the immu-nological and the molecular biological methods to understand the significance of Hsp(hypoxia) ad-aptation in the organic high elevation,through the mammal heat shock response.(1) From high ele-vation to low elevation(natural hypoxia) :Western blot and conventional RT-PCR and real-time fluo-rescence quota PCR were adopted.Expression difference of heat shock protein of 70(Hsp70) and natural expression of brain tissue of Hsp70 gene was determined in the cardiac muscle tissue among the different elevation mammals(yak) .(2) From low elevation to high elevation(hypoxia induction) :The mammals(domestic rabbits) from the low elevation were sent directly to the areas with different high elevations like 2300,3300 and 5000 m above sea level to be raised for a period of 3 weeks be-fore being slaughtered and the genetic inductive expression of the brain tissue of Hsp70 was deter-mined with RT-PCR.The result indicated that all of the mammals at different elevations possessed their heat shock response gene.Hsp70 of the high elevation mammal rose abruptly under stress and might be induced to come into being by high elevation(hypoxia) .The speedy synthesis of Hsp70 in the process of heat shock response is suitable to maintain the cells' normal physiological functions under stress.The Hsp70 has its threshold value.The altitude of 5000 m above sea level is the best condition for the heat shock response,and it starts to reduce when the altitude is over 6000 m above sea level.The Hsp70 production quantity and the cell hypoxia bearing capacity have their direct ratio.
The mammal''s high elevation (hypoxia) adaptation was studied by using theimmu-nological and the molecular biological methods to understand the significance of Hsp (hypoxia)adaptation in the organic high elevation, through the mammal heat shock response. (1) From highelevation to low elevation (natural hypoxia): Western blot and conventional RT-PCR and real-timefluorescence quota PCR were adopted. Expression difference of heat shock protein of 70 (Hsp70) andnatural expression of brain tissue of Hsp70 gene was determined in the cardiac muscle tissue amongthe different elevation mammals (yak). (2)From low elevation to high elevation (hypoxia induction):The mammals (domestic rabbits) from the low elevation were sent directly to the areas with differenthigh elevations like 2300, 3300 and 5000 m above sea level to be raised for a period of 3 weeksbefore being slaughtered and the genetic inductive expression of the brain tissue of Hsp70 wasdetermined with RT-PCR. The result indicated that all ofthe mammals at different elevationspossessed their heat shock response gene. Hsp70 of the high elevation mammal rose abruptly understress and might be induced to come into being by high elevation (hypoxia). The speedy synthesis ofHsp70 in the processof heat shock response is suitable to maintain the cells'' normal physiologicalfunctions under stress. The Hsp70 has its threshold value. The altitude of 5000 m above sea level isthe best condition for the heat shock response, and it starts to reduce when the altitude is over6000 m above sea level. The Hsp70 production quantity and the cell hypoxia bearing capacity havetheir direct ratio.
作者
WANG Xiaolin1,2,3,XU Cunshuan4,WANG Xiujie5,WANG Dongjie6,WANG Qingshang7 & ZHANG Baochen11.Northwest Plateau Biological Research Institute,Chinese Academy of Sciences,Xining 810001,China
2.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China
3.Affiliated Hospital of Medical College of Qinghai University,Xining 810001,China
4.Key Laboratory of Ministry of Science and Technology of China in Henan Normal University,Xinxiang 453000,China
5.Key Laboratory of China Ministry of Education in Oncology Research Institute of Western Hospital of Sichuan University,Chengdu 610041,China
6.Guangzhou Meizheng Pharmaceutical Industry & Key Laboratory of Ministry of Health of China,Guangzhou 510075,China
7.Rubber Research Institute,China Tropical Agricultural Academy of Sciences,Haikou 571737,China
