摘要
Applying the patch-clamp technique to vacuoles from Radish we studied the effects of Cu2+ on Slow Vacuolar (SV) current’s characteristic. Our results show that Cu2+ in bath solution at higher concentration inhibits SV currents and the percentage of inhibition increases with increasing concentration and changes with different voltage. When at lower concentration, Cu2+ significantly promotes the SV currents and the promotion ratio decrease with increasing voltage. At the same time, the time constants of activation become lesser after adding Cu2+. These results show that there may be some Cu2+ binding sites on SV channels and binding to which can change SV current’s characteristic.
Applying the patch-clamp technique to vacuoles from Radish we studied the effects of Cu2+ on Slow Vacuolar (SV) current’s characteristic. Our results show that Cu2+ in bath solution at higher concentration inhibits SV currents and the percentage of inhibition increases with increasing concentration and changes with different voltage. When at lower concentration, Cu2+ significantly promotes the SV currents and the promotion ratio decrease with increasing voltage. At the same time, the time constants of activation become lesser after adding Cu2+. These results show that there may be some Cu2+ binding sites on SV channels and binding to which can change SV current’s characteristic.
基金
support by the National Natural Science Foun-dation of China(Grant No.20637010)
the Shanxi Pro-vincial Natural Science Foundation for Youth(Grant No.20041032).
