摘要
本实验对牛性别鉴定后IVP胚胎的冷冻方法及相关技术进行研究,目的是改善其冷冻效果,提高牛性别鉴定后IVP胚胎的移植妊娠率。实验结果如下:1、性别鉴定后IVP胚胎不能用常规冷冻方法冷冻。用1.5MEG冷冻剂冷冻的胚胎,解冻培养24小时后全部死亡;用美国A、B公司特制的体内半胚胎的次冻保存。2、性别鉴定IVP胚胎用OPS玻璃化冷冻,实验表明,OPS玻璃化冷冻与鲜胚对照组体外培养没有显著差异(P>0.05)。说明OPS玻璃化冷冻牛IVP性别鉴定胚胎是可行的。
The objective of this study was to improve the cryopreservative offect and preganacy rate on bovine sexed IVP embryos .The results as follows :1.the conventional method was not feasible to sexed IVP embryos ,The survival rate of sexed IVP embryos frozen with 1.5M EG was 0% after 24h culture .the viability rate of Embryos frozen with demiembryo cryoprotectant which produced by A.B technology ,was after 24h and 48h cuiture .2. there were no differences between vitrification in combination with OPS method and control which consisted of fresh sexed IVP embryos (P>0.05). It was concluded that bovine sexed IVP embryos were could be successfully frozen by vitrification in combination with OPS methods .
