OPN在肝细胞癌中的表达及其临床意义

The expression of OPN in hepatocellular carcinoma and their clinical significance

目的研究OPN在HCC中的表达与临床病理特征的关系。方法选取48例HCC手术切除的新鲜标本和10例正常肝组织,10%福尔马林固定,常规石蜡包埋,每个标本行连续切片,厚4μm。应用SABC免疫组化法,以OPN单克隆抗体对HCC组织切片进行染色。用X2检验、t检验和Fisher确切概率法,对OPN在HCC中的表达及其临床病理特征的关系进行研究。结果OPN在HCC癌组织中的阳性率为50.00%,明显高于癌旁及正常肝组织(P<0.01)。OPN在高、中、低分化HCC中评分是0.67±1.63、2.14±1.81、2.20±1.74,低分化明显高于高分化(p<0.05)。OPN在有转移与无转移HCC中的评分是2.64±1.84、1.25±1.74,有转移者明显高于无转移者(p<0.01)。OPN与其它病理特征无关。结论OPN在HCC癌组织中的表达明显升高,病理分化差者表达明显升高,且伴有转移者高于不伴转移者,提示OPN可能促进了HCC的发展和转移。

Objective To study the expression of OPN in HCC and the relationship between the expression and clinical pathological characterization.Methods 48 fresh resected specimens from the patients with HCC and 10 normal liver tissue were selected, fixed with 10% formalin, paraffin embed in routine, each sample were proceed serial section, about 4μm thick, tissue section of HCC were stained by SABC immunohistochemistry, with OPN in HCC and the relationship between the expression and clinical pathological characterization were studied with X^2 check, t check and Fisher precise probability.Results the positive rates of OPN in the tissue of HCC was 50.00%, it was high obviously than paracarcinoma tissue and normal liver tissue (P<0.01). the expression of OPN were opposite with MTS, The scores of OPN in well, middle and poorly differentiated HCC were 0.67±1.63、 2.14±1.81 and 2.20±1.74,respectively, the scores of OPN in well-differentiated HCC were significantly lower than those in poorly-differentiated cancer ( p< 0.05).The scores of OPN in HCC with metastatic and without metastatic cancer were 2.64±1.84 and 1.25±1.74 respectively,the scores of OPN in HCC with metastatic cancer were higher significantly than those without metastatic cancer(p<0.01); The expression of OPN were not associated with other pathological characterization.Conclusion The expression of OPN in the tissue of HCC was increased significantly, but the expression decreased significantly in those which pathological degree was well, and the expression of OPN in the tissue of HCC were associated with metastasis or no-metastasis. This showed that OPN promoted possibly the development and metastasis of HCC.