永生化的BMSCs移植促进大鼠脊髓损伤后功能恢复

Recovery of rats spinal cord injury by transplantation of immortalized Bone marrow stem cells

目的探讨神经生长因子(Nervegrowthfactor,NGF)和脑源性神经生长因子(BrainDerivednervegrowthfactor,BDNF)基因修饰后永生化的骨髓基质细胞(Bonemarrowstemcells,BMSCs)移植促进大鼠脊髓损伤后功能恢复。方法SD大鼠制备成脊髓损伤动物模型。随机分为损伤对照组(A组)、BMSCs移植组(B组)和NGF、BDNF基因修饰后永生化的BMSCs移植组(C组)。治疗后2、4、6周,每组动物分别进行联合行为评分(GBS)、运动诱发电位(MEP)、感觉诱发电位(SEP)检查、双下肢功能测定(爬坡试验),评价脊髓损伤功能恢复情况。结果随时间的延长,B组GBS、MEP、SEP及下肢功能明显改善,与其它组相比较,差异有显著性,P<0.05。结论NGF、BDNF基因修饰后永生化的BMSCs移植能恢复损伤脊髓的功能。

Objective To study Recovery of rats spinal cord injury by transplantation on. immortalized Bone marrowe stem cells(BMSCs). Methods Spinal cord injured rats were divided into 3 goaeps. Group C consisted of the rat with treated with the transplantation of immortalized BMSCs modified by NGF?BDNF genes; group B of the rats with transplantation of BMSCs without immortalized; group A of the rats with without treatment At 2?4?6weeks the rats were examined with GBS score and neurological function were tested. Results The tintracord transplantation of immortalized BMSCs group were, improved the GBS score and neurological outcome of injured spinal cord, Conclusions The transplantation of immortalized BMSCs can improve the recovery of Spinal cord injured on rats.