肾康注射液对梗阻性大鼠肾间质NF-КB和TGF-β1表达影响的研究

Study of shenkang injection on the expression of nuclear factor-КB and TGF-β1 in obstruction rats

目的研究肾康注射液对梗阻性大鼠肾间质NF-КB和TGF-β1的表达情况的影响,为临床用药提供依据。方法采用单侧输尿管结扎(UUO)肾间质纤维化模型,将Wistar大鼠72只随机分为三组:假手术组、模型组和治疗组。于术后第5天、第10天、第15天,处死各组大鼠8只,在光镜下观察肾脏组织病理改变,并应用免疫组织化学方法检测肾小管间质中NF-КB和TGF-β1的表达情况。结果同模型组相比,治疗组可明显降低NF-КB和TGF-β1在肾间质中的表达。结论肾康注射液可通过降低NF-КB和TGF-β1在肾间质中的表达,抑制肾间质纤维化,从而具有延缓慢性肾功能衰竭的作用。

Objective To investigate shenkang injection on the expression of nuclear factor-КB and TGF-β1 in rats with renal interstitial fibrosis. Methods Rats unilateral ureteral obstruction of (UUO) model was used for the study. Seventy-two Wistar rats were randomly divided into sham operation ,unilateral obstruction and shenkang injection treated groups, and were sacrificed on day 5,10,15 postoperatively. The sites and levels of NF-КB and TGF-β1 were detected by immunohistochemistry staining. Results The levels of NF-КB and TGF-β1 in the UUO groups significantly increased compared to the sham-operation groups (P<0.05) and paralleled with the degree of renal interstitial fibrosis. In comparison with the model group , the levels of NF-КB and TGF-β1 were significantly reduced by shenkang injection treatment . Conclusions Shenkang injection could retard the expression of NF-КB and TGF-β1 in renal interstitial fibrosis and inhibit the chronic renal failure in some extent.