摘要
目的:探讨乌梅丸抗肝纤维化的作用机理。方法:将105只小鼠随机分为空白对照组、模型对照组、小柴胡汤组、秋水仙碱组和乌梅丸大剂量、中剂量、小剂量组,除空白对照组外,其余各组用猪血清诱导免疫损伤性大鼠肝纤维化后,分别灌胃给药,采用原位杂交法测定肝组织α1(Ⅰ)型前胶原mRNA。结果:空白对照组mRNA表达低于其他各组(P<0．05),乌梅丸各组α1(Ⅰ)型前胶原mRNA的表达低于模型组和秋水仙碱组、小柴胡汤组(P<0．05)。结论:乌梅丸通过减少Ⅰ型胶原的形成,具有明显的抗免疫损伤性大鼠肝纤维化作用。
Objective: To investigate the mechanism of Wumei pill in treating hepatic anti-fibrosis. Methods: 105 rats were divided into blank group, model group, Xiao Chaihu decoction group, colchicines group and high/middle/low dosage of Wumei groups. Except for blank group, the pig serum was used to induce the immunity lesion of hepatic fibrosis in rats in all other groups, and then, the medicine was filled into the stomach. mRNA ofα1(Ⅰ) procollagen in hepatic tissue was measured with situ hybridization. Result: The expression of mRNA in blank group was lower than that in other groups(P<0.05); the expression ofα1Ⅰprocollagen mRNA in each Wumei groups were lower than that in all other groups. Conclusion: Wumei pill is very effective to protect liver from immunity lesion hepetic fibrosis by redule the contento ofⅠprocollagen in rats.
出处
《世界中西医结合杂志》
2006年第1期19-21,共3页
World Journal of Integrated Traditional and Western Medicine
基金
河南省教育厅科研基金课题
NO:2003360185。
