脑清灵汤治疗抽动秽语综合征的作用及机理研究

Study on Function and Mechanism of Nao Qingling Tang (Nao Qingling Decoction) for the Treatment of Gilles de la Tourette syndrome

目的:阐明脑清灵汤治疗抽动秽语综合征(TS)的作用及作用机理。方法:84只健康昆明种小鼠随机分为6组,即正常对照组,模型组,脑清灵汤高、中、低剂量组,阳性药对照组,每组14只动物,采用苯丙胺诱发小鼠中枢神经系统亢进法建立拟TS模型,观察小鼠定型活动和自主活动情况,并采用荧光分光光度法测定脑内单胺类神经递质如多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)及其代谢产物5-羟吲哚乙酸(5-HIAA)的含量。结果:与模型组比较,脑清灵汤3剂量组和氟哌啶醇组小鼠由苯丙胺诱发的定型活动显著减少(P<0.01);脑清灵汤高、中剂量组和氟哌啶醇组小鼠的自主活动明显减少(P<0.01),低剂量组TS模型小鼠的自主活动也有所减少(P<0.05);并且脑清灵汤3剂量和氟哌啶醇能显著降低小鼠纹状体中DA、NE含量(P<0.01);脑清灵汤高、中剂量组小鼠脑内5-HT及5-HIAA的含量与模型组相比有显著性降低(P<0.01),而低剂量组与氟哌啶醇组小鼠脑内5-HT及5-HIAA的含量与模型组相比无显著性差异(P>0.05)。结论:脑清灵汤对TS动物模型有治疗作用,其作用机理可能与其影响脑内单胺类神经递质的分泌代谢有关。

Objective:To elucidate the function and mechanism of Nao Qingling Tang (Nao Qingling Decoction) for the Treatment of Gilles de la Tourette syndrome.Methods: 84 Kunming mice were randomly divided into 6 groups,namely,the normal control group,model group,positive control group(treated by haloperidol),and Nao Qingling Tang (Nao Qingling Decoction) treated groups of three kinds of dosage,which was 20g/kg,15g/kg and 10g/kg respectively.14 animals in each group.The model of experimental tourette syndrome(ETS) was established by stimulating central nervous system of mice with AP,then the stereotypia and autonomic activities of mice were observed,and fluorophotometry was used to determine the levels of monoamine neurotransmitters such as dopamine(DA),norepinephrine(NE),5-hrdroxytryptamine(5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) in the brains.Results: Compared with that of model group,AP induced stereotypia of mice in Nao Qingling Tang (Nao Qingling Decoction) treated groups and positive control group were obviously relieved(P<0.01).Autonomic activities of mice in Nao Qingling Tang (Nao Qingling Decoction) treated groups of dosage of 20g/kg and 15g/kg and positive control group were significantly reduced(P<0.01),and that was reduced in mice of Nao Qingling Tang (Nao Qingling Decoction) treated group of dosage of 10g/kg too(P<0.05).Moreover,the content of DA and NE in corpora striata of mice in Nao Qingling Tang (Nao Qingling Decoction) treated groups and positive control group were obviously reduced(P<0.01).The content of 5-HT and 5-HIAA of mice in Nao Qingling Tang (Nao Qingling Decoction) treated groups of dosage of 20g/kg and 15g/kg were significantly reduced(P<0.01),while those in Nao Qingling Tang (Nao Qingling Decoction) treated group of dosage of 10g/kg and positive control group didn’t show significant changes.Conclusion: Nao Qingling Tang (Nao Qingling Decoction) has therapeutical effect on ETS model and its functionary mechanism may be related with its effects on secretion or metabolism of neurotransmitter in the brains.