血管内皮生长因子作为结直肠癌新生血管标志的临床价值

Clinical value of vascular endothelial growth factor as a marker of angiogenesis in colorectal cancer

目的探讨血管内皮生长因子(VEGF)在结直肠癌组织中的表达及其作为肿瘤新生血管标志的临床价值。方法采用免疫人VEGF单克隆抗体及鼠抗人因子Ⅷ相关抗原(F-8RAg)单克隆抗体,通过免疫组化技术对48例结直肠癌手术切除的癌组织及癌旁正常组织(对照组织)中VEGF和F-8RAg的表达进行检测。结果结直肠癌组织VEGF表达阳性率为62.5%(30/48),对照组为16.7%(8/48),χ2=64.352,P<0.01。结直肠癌组织F-8RAg标记的MVD为100.9±16.0,对照组为46.8±11.9,t=18.351,P<0.01。VEGF的表达与大肠癌的淋巴结转移(P<0.01)、远处转移(P<0.05)及临床分期有关(P=0.01);而F-8RAg标记的MVD与大肠癌的淋巴结转移、远处转移及临床分期无明显相关(P>0.05)。VEGF在结肠癌组织的表达与F-8RAg的表达无相关性(P>0.05)。结论VEGF在结直肠癌组织新生血管有良好表达,可以作为结直肠癌新生血管有价值的标志。

Objective To determine the expression of vascular endothelial growth factor (VEGF) in colorectal cancer and its clinical value as a marker of neoplasm-related angiogenesis. Methods Samples of colorecatal cancer and adjacent normal (control) tissues were obtained from 48 patients with colorectal cancer, and stained with anti-VEGF monocolonal antibody (mAb) or anti-factor VIII-related antigen mAb (anti-FVIII-RAg mAb). The expression of VEGF and FVIII-RAg in the samples was detected by immunohistochemistry. Results The positive rate of VEGF in colorectal cancer tissue was 62.5% (30/48), significantly higher than that of control tissue [16.7% (8/48), χ2=64.352, P<0.01]. The MVD of colorectal cancer with F-8 RAg marker (100.9 ± 16.0) was significantly greater than that of control group (46.8 ± 11.9, t =18.351, P<0.01). The expression of VEGF was significantly correlated with lymph node metastasis (P<0.01), distant metastasis (P<0.05) and Dukes stages (P=0.01); but MVD had no significant correlation with them (P>0.05). There was no significant correlation between the expression of VEGF and F-8 RAg in colorectal cancer tissue (P>0.05). Conclusions VEGF is highly expressed in the vascular endothelium of neoplasm-related microvessels of colorecatal cancer. It can be used as a marker of angiogenesis in colorecatal cancer.