亚硒酸钠对实验性自身免疫性甲状腺炎大鼠甲状腺细胞凋亡的影响

Effects of sodium selenite on apoptosis of thyrocytes in rats with experimental autoimmune thyroiditis

目的探讨亚硒酸钠对实验性自身免疫性甲状腺炎(EAT)大鼠甲状腺细胞凋亡的影响。方法Wistar大鼠分为正常对照组、自身免疫性甲状腺炎(EAT)组和硒干预EAT组。制备EAT大鼠模型,硒干预EAT组给予亚硒酸钠灌胃。用放射免疫法(RIA)测定各组大鼠自身抗体水平,HE染色观察甲状腺组织病理改变及TUNEL法标记甲状腺组织中凋亡细胞,观察甲状腺细胞凋亡情况。结果正常对照组、EAT组、硒干预EAT组的TgAb分别(6.94±1.13)%、(36.24±3.64)%、(17.23±2.90)%,TmAb分别为(5.96±1.40)%、(27.12±5.06)%、(15.98±2.45)%。硒干预EAT组TgAb、TmAb水平较EAT组明显下降(P<0.05)。各组大鼠甲状腺组织炎症程度及凋亡程度评分结果比较显示,不同组别的甲状腺组织病变程度间差别有显著性(P<0.001),硒干预EAT组的甲状腺滤泡破坏程度较EAT组减轻,淋巴细胞浸润减少(P<0.05),甲状腺细胞的凋亡数量较EAT组明显减少(P<0.05)。结论亚硒酸钠可能通过抑制甲状腺细胞的凋亡来减轻或抑制自身免疫性甲状腺炎的免疫损伤。

Objective To investigate the effects of sodium selenite on the apoptosis in thyroid tissues of rats with autoimmune thyroiditis.Methods Wistar rats were divided into three groups: normal control,experimental autoimmune throiditis(EAT) group,and selenium prophylaxis EAT group.EAT model was established by high-dose iodine intake and pig thyroglobulin injection.Rats in selenium prophylaxis EAT group were given a certain amount of sodium selenite(0.2mg/kg) by intragastric administration.The levels of autoantibo...