摘要
利用高效液相色谱建立了中药青蒿中青蒿素、芦丁、异槲皮苷、水杨酸、槲皮苷、槲皮素6种成分同时定量的分析方法,其中线性范围宽、相对标准偏差低、精密度高、重现性好。色谱最佳条件是:Tiahhe Kromasil C18 100 A(5μm,250 mm×4.6 mm)反相色谱柱;紫外检测波长:279 nm;流动相:V(甲醇)∶V(水)=50∶50(含0.02%三氟乙酸);流速:0.5 ml/min;柱温:30℃。同时与蒸发光散射检测器相比较,除青蒿素外其它成分检测灵敏度高。
A general method to separate and quantify simultaneously 6 active ingredients(arteannuin,rutin,isoquercitrin,salicylic acid,quercitrin,quercetin) in traditional Chinese medicine Artemisia annua L.was established by high performance liquid chromatography(HPLC).This method had a wide linear range,low relative standard deviation,high precision and perfect reproducibility.Optimized apparatus condition was explored as followings An Tiahhe Kromasil C18 100 A(5 μm,250 mm×4.6 mm) reversed-phase column was applied u...
出处
《四川大学学报（工程科学版）》
EI
CAS
CSCD
北大核心
2009年第4期119-124,共6页
Journal of Sichuan University (Engineering Science Edition)
基金
国家自然科学基金资助项目(29877018)
关键词
青蒿素
同时定量分析
高效液相色谱法
arteannuin
simultaneous quantitative analysis
high performance liquid chromatography
