摘要
目的:探讨RASSF1A基因在肝细胞肝癌(简称肝癌)组织中的表达情况并探讨其临床意义。方法:采用逆转录-聚合酶链反应(RT-PCR)法检测48例肝癌组织及癌旁正常组织RASSF1A基因的转录水平。结果:48例肝癌组织中有32例RASSF1AmRNA表达缺失;但48例相应癌旁组织仅有16例RASSF1AmRNA表达缺失。Logistic逐步回归分析表明患者的年龄、性别、肝功能、血AFP、肝癌分化、肝癌大小、肝癌有无包膜与RASSF1AmRNA是否表达无显著相关性(P>0.05)。但在HBsAg(+)患者的肝癌组织及相应的癌旁组织中RASSF1AmRNA的表达率显著低于HBsAg(-)患者(P<0.01,P<0.05)。结论:RASSF1A表达缺失在肝癌的发生、发展中起重要的作用。
Objective:To study the expression of RASSF1A gene in hepatocellular carcinoma(HCC) tissues and to investigate its clinical roles.Methods:Reverse transcription-polymerase chain reaction(RT-PCR) was used to detect the mRNA expression of RASSF1A in the tumor samples and non-cancerous liver samples obtained during operation from 48 HCC patients.Results:No expression of RASSF1A in HCC was observed in 32 of 48 cases;in contrast, RASSF1A was expressed in 16 of 48 non-cancerous liver samples.Using the multivariable...
出处
《南通大学学报（医学版）》
2009年第5期339-340,共2页
Journal of Nantong University(Medical sciences)
关键词
基因表达
肝细胞肝癌
RASSF1A
逆转录—聚合酶链反应
Gene expression
Hepatocellular carcinoma
RASSF1A
Reverse transcription-polymerase chain reaction
