氧、糖剥夺预处理对大鼠海马神经元热休克蛋白70、c-fos表达的影响

Expression of HSP70,c-fos in rat hippocampal neurons after oxygen and glucose deprivation preconditioning in vitro

目的:观察氧、糖剥夺预处理对体外培养大鼠海马神经元热休克蛋白70(HSP70)、c-fos基因表达的影响,探讨预处理的神经保护机制。方法:取新生SD大鼠海马组织神经元进行原代培养,培养至12d时,随机分为4组:对照组,单纯氧、糖剥夺组,氧、糖剥夺预处理组和致死性氧、糖剥夺组(n=30)。以换用无糖Earle’s液培养作缺糖处理,通入含体积分数为85%N2、含体积分数为10%H2和含体积分数为5%CO2混合气体作缺氧处理,各组均采用相应的处理方法。应用免疫细胞化学SP法检测各组神经元HSP70及c-fos的表达。结果:HSP70及c-fos的表达在4组比较差异有统计学意义(F=31.76,50.44,P<0.05);氧、糖剥夺预处理组HSP70及c-fos的表达较对照组、单纯氧、糖剥夺组和致死性氧、糖剥夺组显著增加。结论:氧、糖剥夺预处理可能通过诱导HSP70及c-fos的表达,发挥其神经保护作用。

Aim:To observe the expression of HSP70,c-fos in rat cortical neuron cultured in vitro after oxygen and glucose deprivation preconditioning.Methods:Hippocampal neurons from new-born rat were cultured and randomly allocated into the following groups:control group,oxygen and glucose deprivation group,oxygen and glucose deprivation preconditioning group,oxygen-glucose deprivation causing death group(n=30).The expression of HSP70 and c-fos in four groups was measured by using immunocytochemical technique.Results...