中棉所12的黄萎病抗性遗传与育种应用研究

Studies on the Inheritance of Verticillium wilt Resistance in CCRI 12 and its Utilization in Cotton Breeding

以2个海岛棉品种和5个陆地棉品种为材料与中棉所12进行正反交,配制14个杂交组合的F1和F2。采用纸钵育苗,撕底伤根接种方法对14个组合的F1和F2群体进行黄萎病抗性鉴定。结果表明,以中棉所12作父本与海岛棉抗黄萎病品种或陆地棉抗黄萎病品种进行杂交,F2抗(耐)病株与感病株的分离符合3:1的分离规律,说明海岛棉的抗黄萎病性对于中棉所12的耐黄萎病性为显性,中棉所12的耐黄萎病性对于陆地棉的感黄萎病性为显性,控制黄萎病抗性的基因为一个显性主基因。然而,以中棉所12为母本与海岛棉品种、抗病陆地棉品种和感病陆地棉品种进行杂交,F2群体中90%以上的个体为抗病类型,说明中棉所12的细胞质中存在着抗黄萎病的遗传成分,具有细胞质母体遗传的特点,在棉花抗黄萎病育种中具有重要的利用价值。

Two cultivars of Gossypium barbadense and five upland cotton(G.hirsutum) cultivars were used to cross with CCRI 12,and 14 hybrids and its F1 and F2 populations were produced.The inheritance and its utilization of CCRI 12 in breeding program for Verticillium wilt resistance were studied in this experiment,using the method of seedling inoculation.The results showed that,for the combinations used CCRI 12 as paternal parent,the ratio of the resistant and susceptible seedlings in F2 were done according with the ...