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PINCH-1因子在白血病骨髓基质细胞中的表达及意义 被引量：1

The expression of PINCH-1 in leukemia bone marrow stromal cells and its significance
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摘要 目的研究PINCH-1因子在急性白血病骨髓基质细胞中的表达及意义。方法培养骨髓基质细胞,用West-ern-blotting和RT-PCR观察PINCH-1 mRNA和蛋白的表达情况;进行免疫细胞化学和免疫荧光染色,观察PINCH-1蛋白的表达及其在细胞内的分布情况。结果在急性淋巴细胞白血病和急性髓系白血病患者骨髓基质细胞中,PINCH-1在mRNA水平和蛋白水平均比正常骨髓基质细胞增高(P<0.01);急性淋巴白血病与急性髓系白血病之间PINCH-1蛋白的表达有显著差异(P<0.01),但mRNA表达无明显差别。结论PINCH-1在急性白血病骨髓基质细胞表达明显高于正常骨髓基质细胞,可能与急性白血病发生发展相关。 Objective To investigate the expression of PINCH-1 in acute leukemia bone marrow stromal cells and its significance. Methods Bone marrow stromal cell were cultivated. The expression of PINCH-1 mRNA and protein were observed by RT-PCR and Western blotting; the distribution and expression of PINCH-1 protein in target cells were observed by immunocytochemistry and immunofluorescence technique. Results The levels of PINCH-1 mRNA and protein were significant higher in bone marrow stromal cells of patients with a...
作者 徐葳 曾东风 孔佩艳 梁雪 王金良 张江 彭贤贵
机构地区 第三军医大学新桥医院血液科
出处 《免疫学杂志》 CAS CSCD 北大核心 2009年第6期701-704,共4页 Immunological Journal
基金 第三军医大学创新课题资助(2007XG33)
关键词 PINCH-1 骨髓基质细胞 白血病 PINCH-1 bone marrow stromal cells leukemia
分类号 R733.7 [医药卫生—肿瘤]
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  • 1Jurata LW, Gill GN. Structure and function of LIM domains[J]. Curr Top Microbiol Immunol, 1998, 228(4): 75-113.
  • 2Bach I. The LIM domain: regulation by assoeiation[J]. Mech Dev,2000,91 (3) : 5-17.
  • 3Tu Y, Li F, Goicoechea S, et al. The LIM-only protein PINCH directly interacts with intergrin-linked kinase and is recruited to integrin-rich sites in spreading cells[J]. Mol cell Biol, 1999, 19(3): 2425-2434.
  • 4Jessica W-R, Anna D, Ghazwan M, et al. The signaling adapter protein PINCH is up-regulated in the stroma of common cancer, notably at invasive edges[J]. Cancer, 2002, 95 (6) :1387-1395.
  • 5Velyvis A, Yang YW, Wu CY,et al. Solution structure of the focal adhesion adaptor PINCH LIM1 domain and characterization of its interaction with the integrin-linked kinase ankyrin repeat domain[J]. J Bid Chem, 2001, 276(6): 4932-4939.
  • 6Tu Y, Li F, Wu C. Nck-2, a novel Src homology2/3-comtaining adaptor protein that interacts with the LIM-only protein PINCH and components of growth factor receptor kinase-signaling pathways[J]. Mol Biol cell, 1998, 9(4): 3367-3382.
  • 7Wu C, Keightley SY, Leung-Hagesteijn C, et al.Integrinlinked protein kinase regulate, fibroneetin matrix assembly, E-Cadherin expression , and tumorigernieity [J]. J Biol Chem ,1998, 273(5): 528-536.
  • 8Armstrong PB, Armstnong MT. Intercellular invasion and the organizational stability of tissues:a role for fibronectin[J].Biochim Biophys ACTA, 2000, 1470(4):9-20.
  • 9Beindt A, Borsi L, Hyckel P, et al. Fibrillary co-deposition of laminin-5 and lorige unspliced tenascin-C in the invasive front of oral squamous cell carcinoma in vivo and in vitro [J]. J Cancer Res Clin Oncol , 2001 , 127(4) : 286-292.
  • 10O' Donnell JL, Joyce MR, Shannon AM, et al. Oncological implications of hypoxia inducible factor- 1 alpha ( HIF- 1 alpha) expression[ J]. Cancer Treat Rev, 2006, 32 (6) : 407 - 416.

共引文献9

同被引文献10

  • 1李晓青,吕跃.骨髓基质细胞与白血病的关系及其在基因治疗中的研究进展[J].癌症,2005,24(8):1033-1036. 被引量：3
  • 2Ezoe S,Matsumura I,Satoh Y,et al.Cell Cycle.regulation in hematopoietic stem/progenitor cells[J].Cell Cycle,2004,3(3):326-332.
  • 3Chuanyue WU.PINCH,N(i)ck and the ILK:network wiring at cell-matrix adhesions[J].Trends Cell Biol,2005,15(9):460-466.
  • 4Tu Y,Li F,Goicoechea S,et al.The LIM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells[J].Mol Cell Biol,1999,19(3):2425-2434.
  • 5Dougherty GW,Jose C,Gimona M,et al.The Rsu-1-PINCH1-ILK complex is regulated by Ras activation in tumor cells[J].Eur J Cell Biol,2008,87(8/9):721-734.
  • 6Chen K,Tu Y,Zhang Y.PINCH-1 regulates the ERK-Bim pathway and contributes to apoptosis resistance in cancer cells[J].J Biol Chem,2008,283(5):2508-2517.
  • 7Tiemann K,Rossi JJ.RNAi-based therapeutics-current status,challenges and prospects[J].EMBO Mol Med,2009,1(3):142-151.
  • 8Meade BR,Dowdy SF.The road to therapeutic RNA interference(RNAi):Tackling the 800 pound siRNA delivery gorilla[J].Discov Med,2009,8(43):253-256.
  • 9Ito S,Takahara Y,Hyodo T,et al.The roles of two distinct regions of PINCH-1 in the regulation of cell attachment and spreading[J].Mol Biol Cell,2010,21(23):4120-4129.
  • 10Towne C,Aebischer P.Lentiviral and adeno-associated vector-based therapy for motor neuron disease through RNAi[J].Methods Mol Biol,2009,555:87-108.

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免疫学杂志
2009年 第6期

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