婴幼儿人巨细胞病毒感染154例PCR与ELISA检测比较

Comparison of PCR and ELISA in detecting 154 cases children HCMV infection

目的:探讨采用不同的定量方法及不同的标本检测婴幼儿人巨细胞病毒(HCMV)感染阳性率。方法:采用荧光定量PCR法和ELISA定量法分别对154例疑感染HCMV住院患儿进行HCMV DNA和HCMV IgM抗体的定量检测,其中对73例患儿的血及尿标本同时进行HCMV DNA的检测。结果:血和尿HCMV DNA同时检测,尿检出率明显高于血;血和尿HCMVDNA检出率明显高于血清HCMV IgM;两种方法的联合检测进一步提高阳性率。结论:两种方法同时检测可提高HCMV的检出率;尿标本HCMV DNA的检出率明显高于血标本,采用尿标本更方便,且阳性率高。

Objective:To explore the positive rate of human cytomegalovirus(HCMV) infection in children using different quantitative methods and different specimens.Methods:Fluorescent quantitative PCR and quantitative ELISA method were applied to154 hospitalized children who were suspected to be infected by HCMV,to detect the HCMV DNA and HCMV IgM respectively.Among them,73 children's urine and blood samples were collected simultaneously to detect the HCMV DNA.Results:HCMV DNA positive rate was higher than HCMV IgM si...