利奈唑胺与万古霉素对金黄色葡萄球菌耐药突变选择的比较

Comparison of linezolid and vancomycin in preventing the drug resistant mutants for staphylococcus aureus

目的在体外测定利奈唑胺与万古霉素对金黄色葡萄球菌ATCC29213的最低抑菌浓度(MIC)、防细菌耐药突变体选择浓度(MPC)和突变选择指数(SI),比较其防耐药突变能力。方法采用肉汤法富集1×1010CFU/mL金黄色葡萄球菌ATCC29213,采用平板稀释法测两种药物对金黄色葡萄菌的MIC、MPC以及SI,同时测定万古霉素联合利福平与左氧氟沙星后的MPC、SI。结果利奈唑胺与万古霉素对金黄色葡萄球菌ATCC29213的MIC、MPC以及SI分别为1.01、.0 g/L;6.4、51.2 g/L;6.4、51.2;万古霉素分别联合利福霉素与左氧氟沙星后的MPC及SI分别为12.8 g/L2、5.6;8.0 g/L、16.0。结论利奈唑胺对金黄色葡萄球菌防耐药突变能力强于万古霉素,万古霉素与利福霉素及左氧氟沙星联合后均能降低万古霉素的MPC、SI,提高万古霉素的防耐药突变能力。

Objective To determine the minimum inhibitory concentration(MIC),mutant prevention concentration(MPC) and selection index(SI) of linezolid and vancomycin for staphylococcus aureus in vitro and compare their capacity for restricting the selection of staphylococcus aureus resistant mutants.Methods The staphylococcus aureus strain ATCC25923 was enriched in broth,and the bacterial concentration was adjusted to 1010 colony forming units per milliliter.MIC,MPC and MPC of linezolid and vancomycin for staphylococcu...