羟基红花黄色素A对四氯化碳急性肝损伤的保护作用

Protective effect of hydroxysafflor yellow A on carbon tetrachloride-induced hepatotoxicity

目的探讨羟基红花黄色素A(HSYA)对四氯化碳(CCl4)急性肝损伤的保护作用。方法将60只昆明种小鼠随机分为正常对照组和CCl4损伤组、HSYA保护组(2、4、8、16 mg/kg组),每组各10只。CCl4腹腔注射建立小鼠急性肝损伤模型。观察肝组织病理学改变;测定血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的活性,肝组织匀浆谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)的含量及肝细胞色素P450 2E1(Cytochrome P450 2E1,CYP2E1)蛋白的表达。结果与CCl4损伤组比较,HSYA保护组肝组织病理改变明显减轻;血清ALT、AST活性明显降低(P<0.05);肝组织匀浆中GSH、SOD含量明显升高(P<0.05);MDA、TNF-α含量明显降低(P<0.05);CYP2E1蛋白表达量增加。综合各指标表明,以8 mg/kg保护组效果最佳。结论HSYA对CCl4急性肝损伤有明显保护作用,其机制可能与抑制脂质过氧化,清除自由基,降低TNF-α水平有关。

Objective To investigate the protective effect of Hydroxysafflor Yellow A(HSYA) against Carbon tetrachloride(CCl4)-induced hepatotoxicity and its mechanism.Methods A total of 60 mice were randomly and averagely divided into 6 groups: the blank control group,the CCl4 group,four dosages of HSYA groups (2,4,8,16 mg/kg).10% CCl4 was used to induce acute liver injury in all groups expect for the control group.All animals were sacrificed after 24h from CCl4 intraperitoneal injection.Liver injury was assessed by e...