铝螯合剂对染铝大鼠心脏毒性的保护作用

Protection of aluminum chelating agent against cardiac toxicity in rats exposed to aluminum chloride

目的研究氯化铝(AlCl3)染毒对大鼠的心脏毒性及铝螯合剂1,2-二甲基-3-羟基-4-吡啶酮(DFP)的保护作用。方法35只雄性Wistar大鼠,按体质量随机分为阴性对照组、铝染毒组及DFP低、中、高剂量组。AlCl3172.5mg/(kg.d)染毒8周后分别给予不同剂量的DFP2周,测定血清中乳酸脱氢酶(LDH)、肌酸激酶(CK)、谷草转氨酶(AST)的活力,心肌组织中超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力及脂质过氧化产物丙二醛(MDA)的含量,心肌组织中铝、锌、铜、铁、钙、镁的含量。结果铝染毒组大鼠血清LDH、CK的活力及心肌中MDA的含量均明显高于阴性对照组(P<0.01),心肌铁含量及GSH-Px的活力低于阴性对照组(P<0.05,P<0.01);DFP低、中、高剂量组中LDH、GSH-Px的活力低于铝染毒组(P<0.01),高剂量组CK的活力低于铝染毒组(P<0.05);且高剂量组各项指标与阴性对照组相比差异无统计学意义(P>0.05);血清AST的活力及心肌铜、锌、钙、镁的含量在各组间比较差异无统计学意义(P>0.05)。结论AlCl3可致大鼠心肌损伤,铝螯合剂DFP在排铝的同时,不影响心肌中必需元素的含量,改善AlCl3所致的心肌损伤。

Objective To explore cardiac toxicity in rats exposed to aluminum chloride(AlCl3),and to evaluate the protection of 1,2-dimethyl-3-hydroxy-4-pyridone(DFP) against toxicity.Methods 35 male Wistar rats were divided into five groups by weight.Four groups except for the negative control group were administered aluminum chloride(172.5 mg/(kg·d)) for 8 weeks,then different dosages of DFP were given for 2 weeks.Activity of LDH,CK and AST in the serum,activity of T-SOD and GSH-Px and content of essential elements w...