干细胞标记物LGR5在结直肠癌发生发展中的表达及意义

Expression and significance of stem cell marker LGR5 proteins in the development and progression of human colorectal carcinoma

目的探讨干细胞标记物LGR5(富含亮氨酸重复单位的G蛋白偶联受体5)蛋白在人结直肠癌组织中的表达水平及其与临床病理特征、结直肠癌演进过程的关系和意义。方法采用免疫组化法分别检测10例结直肠正常组织、59例腺瘤组织、70例结直肠癌标本中LGR5的表达,并分析其与临床病理指标的关系。结果①LGR5在结直肠肿瘤中表达水平明显高于结直肠正常组织,亦明显高于结直肠腺瘤(P<0.05);②结直肠癌组织中LGR5的表达水平与Dukes分期、淋巴结转移呈正相关,与组织分型、分化程度、临床特征无相关性;③结直肠腺瘤中LGR5的表达水平与临床病理参数均无相关性。结论LGR5的表达说明结直肠癌的形成与干细胞相关,LGR5在肿瘤发生过程中可能起一定作用,且与肿瘤的进展相关,可以作为早期诊断的分子标记物。

Objective To investigate expression of stem cell marker LGR5 protein in colorectal cancer(CRC) and to evaluate the relationship between LGR5 and clinico-pathological characteristics of CRC.Methods LGR5 expression was immunohistochemically determined on paraffin-embedded,formalin-fixed biopsy tissues in a total of 139 cases(10 normal colorectal mucosa(NCM),59 colorectal adenoma(CRA) and 70 CRC).Immunoreactivity of CRA and CRC to the molecule was semi-quantified by two pathologists in a double-blinded fashion...