谷胱甘肽对KLE与HEC-1-b子宫内膜癌细胞生长的影响

Effect of glutathione on proliferation of KLE and HEC-1-b endometrial cancer cells

目的比较还原型谷胱甘肽(GSH)对KLE和HEC-1-b子宫内膜癌细胞生长的影响,并探讨其机制。方法①用GSH处理KLE和HEC-1-b子宫内膜癌细胞,噻唑蓝(MTT)法检测两型细胞的增殖水平;②用5 mmol/L GSH处理KLE和HEC-1-b子宫内膜癌细胞,流式细胞仪检测细胞内活性氧(ROS)水平,酶标仪检测细胞内GSH和氧化型谷胱甘肽(GSSG)水平,分光光度计检测细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH-Px)活性。结果在不进行处理的情况下,KLE子宫内膜癌细胞较HEC-1-b子宫内膜癌细胞的ROS水平及CAT活性较低,GSH水平、GSH/GSSG比值及SOD和GSH-Px活性较高(P<0.01)。用GSH处理后,KLE子宫内膜癌细胞生长加快,细胞内ROS和GSH水平及GSH/GSSG比值下降,SOD、CAT和GSH-Px活性降低(P<0.01);HEC-1-b子宫内膜癌细胞生长被抑制,细胞内ROS、GSH及GSSG水平下降,GSH/GSSG比值明显升高,CAT和GSH-Px活性降低(P<0.01)。结论GSH对两种子宫内膜癌细胞生长有明显不同的影响,与两种细胞内ROS水平和抗氧化系统活性有密切的关系。

Objective To investigate the effects of glutathione on proliferation of typeⅠand Ⅱ endometrium cancer cells and to explore its related mechanism.Method ① TypeⅠand Ⅱ endometrium cancer cells were challenged by GSH,and proliferation of cells was observed with MTT assay.② TypeⅠor Ⅱ endometrium cancer cells were treated with 5 mmol/L GSH and the cellular ROS,GSH,and GSSG levels and SOD,CAT and GPx activities were determined.Results Without treatment with GSH,typeⅠ endometrium cancer cells,compared with type Ⅱ e...