摘要
目的探讨大麻素受体1(CB1)在四氢大麻酚(Δ9-THC)抑制CA1区长时程抑制(LTD)中的作用。方法在小鼠腹腔注射Δ9-THC(10 mg/kg)或CB1受体的选择性抑制剂SR141716(SR,5 mg/kg)24 h后切片,在海马CA1区记录场电位EPSP。结果①给予低频电刺激(1 Hz 15 min)诱导CA1区LTD,Δ9-THC可显著降低LTD(P<0.01);②Δ9-THC显著降低LTD的效应可被CB1的选择性抑制剂SR所翻转;③在CB1基因敲除的小鼠,Δ9-THC对LTD的效应与溶剂对照组相比无统计学差异(P>0.05)。结论CB1受体介导Δ9-THC抑制离体海马CA1区LTD的作用。
Objective To explore the role of cannabinoid receptor 1(CB1) in THCinduced inhibition of LTD in the CA1 area in vitro.Methods Field excitatory post-synaptic potentials(fEPSP) were recorded in the stratum radiatum of the CA1 area in vitro.Results ①Low frequency stimulation(1 Hz 15 min) induced-LTD in the CA1 area which was significantly attenuated by Δ9-THC;②THC-induced inhibition of LTD was reversed by SR141716(a CB1 selective inhibitor);③ THC-induced inhibition of LTD was not robustly altered in CB1 knocko...
出处
《山东大学学报（医学版）》
CAS
北大核心
2009年第9期17-20,共4页
Journal of Shandong University:Health Sciences
基金
湖北省卫生厅基金资助课题(JX2B77)
湖北省教育厅基金资助课题(Q200613002)
关键词
大麻素受体1
四氢大麻酚
长时程抑制
突触可塑性
海马
小鼠
Cannabinoid receptor 1
Δ9-tetrahydrocannabinol
Long-term depression
Synaptic plasticity
Hippocampus
Mouse
